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SRX25882080: Transcriptome sequencing of Microcystis aeruginosa
1 ILLUMINA (Illumina NovaSeq 6000) run: 16.2M spots, 4.9G bases, 2.5Gb downloads

Design: RNA-seq strand-specific libraries were prepared following the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA, USA), using 5 g of total RNA. Briefly, rRNA removal was performed using the RiboZero rRNA removal kit (Epicenter, WI, USA), and the RNA was then fragmented using fragmentation buffer. Subsequently, cDNA synthesis, end repair, A-base addition, and ligation of the Illumina-indexed adaptors were carried out according to Illuminas protocol. The libraries were size selected for cDNA target fragments of 200 300 bp on 2% Low Range Ultra Agarose, followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification using TBS380, the paired-end libraries were sequenced using the Illumina NovaSeq 6000 sequencing platform (150bp*2, Shanghai BIOZERON Co., Ltd).
Submitted by: Tongji University
Study: Transcriptome sequencing of Microcystis aeruginosa
show Abstracthide Abstract
The sequencing data is used to study the effect of antibiotics on Microcystis aeruginosa (strain PCC 7806) with different growth rates.
Sample: End; Growth rate: 0.05; Parallel sample 2
SAMN43400275 • SRS22485918 • All experiments • All runs
Library:
Name: MA_D0.05-E_2_R1
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 16.2M spots, 4.9G bases, 2.5Gb
Run# of Spots# of BasesSizePublished
SRR3045691016,209,5264.9G2.5Gb2024-08-28

ID:
34944011

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